Hormone measurements can serve as crucial indicators for assessing an individual's physiological state in the context of Neuro-Information Systems (NeuroIS). Understanding hormonal responses to interruptions can provide insights into human cognition, emotion, behavior, and performance. By analyzing hormone levels, researchers can measure the effect of interruptions on an individual's physiological processes, including performance in social interactions or performance in human–computer interaction situations. This nexus of NeuroIS and interruption science provides a unique perspective on how technological interruptions affect the neurophysiological responses of individuals. Building on a previous umbrella review of neurophysiological measurements in interruption science, in the current paper we review completed empirical studies with a focus on the methodological aspects of the hormone measurements in the papers. Specifically, we describe identified studies that applied hormone measurements with saliva or urine samples by detailing the following factors: research objective, research method, sample size, study population, and research results. Finally, to lay a foundation for future research activities based on the paper analysis, this review outlines methodological complexities of hormone measurements at the nexus of interruption science and NeuroIS.