Synthesized Pulsed Laser ZnO as Anti Diabetic Foot Ulcer Pathogenic Bacteria

The current study aimed to evaluate the inhibitory activity of zinc oxide nanoparticles synthesized by pulsed laser ablation (ZnO NPs) against pathogenic bacteria isolated from patients with diabetic foot ulcer in Baghdad Teaching Hospital, Iraq. ZnO NPs were prepared using pulsed laser ablation. Characterization of the biosynthesized ZnoNPs were carried out by using field emission scanning electron microscopy (FESEM), atomic field microscopy (AFM), UV–visible spectroscopy, and X-ray diffraction (XRD). The results indicate the formation of pure ZnO NPs in a spherical shape with an average size ranging from 20 to 90 nm. Fifty swabs of clinical specimens were collected by using sterilized swabs and cultured on different cultural media. The isolated bacteria were identified based on their cultural, microscopical characteristics, and biochemical tests. The diagnosis was confirmed using Vitek2 System. The results indicated that prepared ZnO NPs had a high activity against Gram- positive and Gram-negative bacteria that causing diabetic foot ulcers.Ttheir activity differed according to the bacteria and various laser pulses. The highest diameters of the inhibition zone were (23.7 ± 0.3, 21.3 ± 0.6, 19.3 ± 0.6, 18.8 ± 0.3, 18.8 ± 1.0, 18.7 ± 0.6, 18.5 ± 0.5, 14.8 ± 0.8, 14.7 ± 0.6, 12.7 ± 0.6, 11.7 ± 0.6, and 11.5 ± 0.5) mm against Kocuria kristinae, Proteus vulgari, Sphingomonas paucimobilis, Staphylococcus aureus, Enterococcus faecalis, Proteus penneri, Staphylococcus hominis, Aeromonas hydrophila, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis, respectively for nanoparticles at a laser pulse of 300.