The Efficacy of Biologically Active Substances in Garden Strawberry Propagation Technology in Vitro

The garden strawberry is currently considered to be the most widespread berry crop. Despite the existence of a well-developed in vitro culture propagation methodology, ongoing technological advancements are required to keep pace with the evolving variety and composition of nutrient media. In the cultivation of crops and biotechnology, the regulation of plant growth and development with biologically active substances is crucial due to their economic viability and cost-effectiveness. The expanding range of biologically active substances, both in their pure form and as components of growth regulators, necessitates a thorough investigation of their beneficial physiological effects on plants. This research was conducted from 2022 to 2023 at the scientific and production center for the nursery cultivation of fruit and berry crops in Southern Russia. We examined the impact of biologically active substances within the Modified nutrient medium and Murashige and Skoog medium on the propagation efficiency of garden strawberry in vitro. Using the Modified medium led to an increase in morphometric parameters and enhanced regenerative capabilities of plants compared to the Murashige and Skoog medium. The biologically active substances investigated (tryptophan, aspartic acid, indole-3-acetic acid) stimulated the growth and development of microplants, with morphometric parameters exceeding those of the control. During the rhizogenesis phase, the inclusion of indole-3-acetic acid in the nutrient yielded the highest rooting rates for garden strawberry microplants – an average of 73.8% across the experiment. This rate was comparable to the use of tryptophan, where the difference of 0.5% was found to be statistically insignificant.