The work presents the results of a life cycle analysis study for the case of organic wine production at a local winery in Pieria. The aim of this study is to determine the impacts associated with the entire process of wine production at “Ktima Kourtis” winery. The boundaries of the system incorporate the annual cultivation of the vineyard and includes all phases of the product’s life cycle until its disposal after consumption. Comprehensive calculations of inputs and outputs for a 750 ml bottle (the operational unit in the LCA study) are performed. In order to quantify the impacts, the LCArt Simulations Engine is used. The LCArt Simulations Engine is employed for impact quantification, functioning as a web application developed under the KYKLOS 4.0 project that was funded by the EU Horizon 2020 Programme. The analysis utilizes both real data from the winery and information available in the Ecoinvent database.