Genetics, Toxicological Background, and Environmental Adaptation Processes of Disease: Vector Mosquitoes in the Amazon
摘要
The influence of climate change on Amazonian ecosystems has produced changes in the way of life of several mosquitoes of epidemiological importance. In this context, aspects of the biological and molecular cycle of mosquitoes in the Amazon were studied to develop new vector control strategies that do not cause damage to the associated fauna. In this study, the transcription of lipophorin, vitellogenin genes, and some biological parameters showed significant changes as the climate simulation environments became more severe in relation to the current environment. In addition, assays to determine the mortality, cytotoxicity, and genotoxicity of Ae. aegypti exposed to dillapiole derivatives were carried out as a new alternative to control of this mosquito resistant to certain types of insecticides in certain geographic areas. Mortality and cytotoxicity occurred in larvae exposed to the dillapiole piperidyl and dillapiole isopropyl ether substances and, furthermore, dillapiole ethyl ether caused a high frequency of damage to the DNA of larvae compared to the negative control. The screening of bacteria revealed three strains (End64LB, End82LB, and End84LB) that belong to the phyla Proteobacteria and Firmicutes and have larvicidal potential against Ae. aegypti, suggesting that dillapiole derivatives and these bacteria are promising for the biological control of Ae. aegypti.