Association of TNF-α-308, IL-1b and Glutathione-S-1 Transferases GSTS (M1/T1) Genes Polymorphism with Male Infertility Risk in Egyptian Population

Male infertility (MI) is a condition characterized by failure of the male to make a fertile woman pregnant. About 10 to 30% of infertility is classified as idiopathic infertility. Many genetic studies exposed association between some genotypes and MI. The current study investigated the correlation between gene polymorphisms of Tumor necrosis factor-alpha (TNF-α-308), the interleukin 1b (IL-1b) and glutathione-S-transferases GSTs (M1/T1) and MI in an Egyptian population. This work involved a total 200 participants (100 infertile male patients and 100 healthy fertile males as controls). Genomic DNA of the studied gene variants underwent genotyping using the ARMS-PCR and multiplex PCR technique. Our results indicated the frequencies of the genotyping homozygote AA (37% vs 5%) for TNF-α (G > A; rs1800629) gene variant was significantly associated with an enhanced risk in infertile males versus control group (P < 0.001). The different genetic models including allelic co-dominant model of Genotyping G/G, G/A and A/A in TNF-α*(G > A; rs1800629) gene variant showed significant difference in infertile males versus control subjects (P < 0.001). Genetic models of the Codominant, Dominant and Recessive GSTS (M1/T1) genes were significant in patients versus controls (p < 0.05). IL-1b showed no association with MI (p > 0.05). Our founding concluded the current study might provide an evidence of correlations between TNF-α, GSTs (M1/T1) models and (GSTT1/GSTT1) gene polymorphisms and the risk of MI among Egyptian males. There was no significant association between IL-1b (rs16944; -511C > T) and MI in this study.