Usage of stored biodiesel as fuel in engine may impact engine’s functioning and enduringness. The prolonged storage of biodiesel head towards the fuel degradation and oxidizes into gums and acids. In order to cease the issues associated to degradation and oxidation of fuel, antioxidants are used. Though numerous works disclosed the potential characteristics of antioxidants for its oxidation stability of fuels, merely few works addressed the characteristics of antioxidants on dry, wet and humid conditions. Three antioxidants namely butylated hydroxy toluene, pyrogallol and tert-butylhydroquinone are selected for the study. The antioxidants proportion of 500, 1000 and 2000 parts per million were selected for individual dispersion with microalgae biodiesel using an ultrasonicator apparatus and its influence of water on its oxidation stability was studied for the storage period of 21 weeks. This research disclosed the oxidative stability of biodiesel within its storage conditions (dry, wet and humid) and observed longer stability for dry conditions than wet and humid storage conditions. The loss in stability of fuels was identified not because of fuel oxidation, but also depends on its polarity of antioxidants.