Elimination of Optical Distortions Arising from In Vivo Investigation of the Mouse Brain

An algorithm is proposed for eliminating refractive distortions caused by the oscillating surface of a liquid when studying the brain of a live mouse. Studies like this in mice allow monitoring neuronal activity in a living organism, and the fluid is needed to ensure that the brain remains in its natural environment. However, the presence of fluid flow causes distortions that significantly complicate tracking waves of neuronal activity. The goal of the present work is to remove effects that displace and distort images of individual parts of the brain, and, in fact, bring the entire image to a static picture. The proposed algorithm, based on tracking individual parts of images, gives a 10% improvement in approximation to a static picture compared to the original recording.