Step-by-Step Guide to Building a Diagnostic Model Using MetaboAnalyst

MetaboAnalyst is an online platform for analyzing and interpreting metabolomics data. Its creators, researchers Jianguo Xia and David Wishart, have kindly made it available for free use worldwide and provided constant improvements. The user has at his disposal many algorithms that require in-depth prior knowledge of various machine learning and statistical analysis terms. To streamline the daily utilization of these tools and guide users in developing a diagnostic model using molecular biomarkers, the author has crafted a tutorial. This comprehensive guide details each step with examples, explanations of graph interpretations, and commentary on the essential concepts needed for a successful analysis.