Human Oocyte Vitrification

Vitrification is an ultrarapid, cooling process that produces glass-like (ice crystal-free) solidification. Water is prevented from forming intercellular and intracellular ice crystals during cooling as a result of cellular dehydration and the use of highly concentrated cryoprotectant. Though the potential negative effects of cryopreservation on the oocyte are minimized through vitrification, there are still factors that can negatively impact oocyte quality that translates to diminished embryo developmental potential or subsequently reducing clinical outcomes. In this chapter, we provide a detailed view on laboratory factors and explore directions to ameliorate clinical oocyte vitrification.