The management of infections in exotic, zoo, and wildlife species is crucial for their health and conservation. Veterinarians often encounter complex health issues in wildlife that differ significantly from those in domesticated animals. The increasing popularity of exotic pet animals has also become a significant trend in recent years, driven by a combination of unique pet appeal and expanding global trade networks. The unique biology of wild species and the rise of antimicrobial resistance complicate treatment. Traditional antimicrobial approaches are becoming less effective, highlighting the need for alternative therapies. Antimicrobial photodynamic therapy (aPDT) has emerged as a significant therapeutic option, offering targeted treatment for infectious diseases that minimizes systemic side effects and reduces the risk of resistance development. This chapter presents the evolving role of aPDT in treating infections among these diverse wild animals and discusses its various applications. For fish, we shed light to the possibility that some approaches used in aquaculture targeting bacterial infections and parasitic infestations in fish intended for human consumption can also be applied for ornamental fish tanks for public or private display. In reptiles, it has been applied to manage carapace fractures and treat fibropapillomatosis in sea turtles, as well as infectious stomatitis in snakes. For birds, aPDT has been successfully used to treat pododermatitis in penguins. Although studies in mammals are limited, aPDT has shown potential in addressing dental issues and for treating infected wounds. Overall, aPDT demonstrates versatility and efficacy, making it a valuable adjunct or alternative to conventional antimicrobial treatments in this field. Although relatively new in this context, its potential to address challenges such as antimicrobial resistance and the treatment of infections in wild animals highlights its growing importance.