Vacuum Gripper-Based Practical Method of Gentle Deposition of Living Cells and Its Filter Substrates onto SiN Films for Electron Beam Irradiation Experiments
摘要
Electron beam irradiation of living cells requires very careful preparation of samples which are typically placed onto SiN thin films that form the barrier between the vacuum of the electron gun and the atmosphere. This paper presents the development of a specialized vacuum gripper and a method of gentle deposition of living cells onto a silicon nitride film. The presented method allows for safe and repetitive preparation of samples mitigating the difficulties resulting from the fragile nature of thin SiN films which are prone to destruction if in direct contact with mechanical tools such as tweezers.