Spiral Wire Microplasma Inducing Growth and Viability of Nasal Cell

In medicine, the promising potential of plasma treatment requires the measurement of possible risks and the prevention of cell damage by direct or indirect application of plasma devices. This study aimed to investigate the impact of plasma discharge generated from spiral wire microplasma on cell viability and the delivery of fluorescein isothiocyanate-dextran 150 (FD-150). At discharge voltages of 2, 3, 4 kVp-p, the growth and viability of the cell was investigated. The influence of the optimized discharge at 3 kVp-p was further studied in three different volumes (1, 2, 3 ml) of cell culture medium, as well as the delivery of FD-150 with a molecular weight of 150 kDa into cells. The olfactory dark basal cell (DBC1.2) from mouse nasal septum, similar to the nasal epithelium, was used. Cell viability was evaluated using a cell counter and a hemocytometer, while drug delivery was measured by the fluorescence intensity of a microplate reader. The results revealed an increased cell number and viability for plasma-irradiated samples compared with control samples at an incubation time of 30 min, whereas at 24 h incubation, the results were approximately the same. Similarly, plasma discharge in 1, 2, and 3 ml volumes of Dulbecco’s Modified Eagle Medium (DMEM) revealed no cell damage by plasma but rather a 3%, 4%, and 5% increase compared with control cells. The results observed for delivery of the drug sample also indicate that the microplasma not only prevent epithelial cell damage, but also increase drug absorption in cells.