Staining and Coverslipping

Staining and coverslippingCoverslipping are the final steps in tissue processing that are required before slide interpretation can be performed by the Mohs surgeon. StainingStaining is the process used to impart differential coloration onto cellular components in order to delineate different cells and tissue structures for microscopic inspectionInspection. HematoxylinHematoxylin and eosinEosin (H&E) stainingStaining is the most common stain used in Mohs Micrographic Surgery and is used to contrast nuclei and cytoplasm in blue and pink colors, and Toluidine Blue (T. Blue)Toluidine Blue (T. Blue) is an alternative stain that highlights tumor stroma. Coverslipping refers to the process of mounting a glass cover over a microscope slide to protect and preserve the tissue sections after stainingStaining is complete. Staining and coverslippingCoverslipping are both essential steps that allow for accurate histologic examination and thus successful Mohs surgery.