Targeting Induced Local Lesions in Genomes: A Transgenic Approach for the Improvement of Desirable Crop in the Current Era of the Changing Climate

TILLING (Targeting Induced Local Lesions in Genomes) was first shown in 2000 as an anti-hereditary special tool that strengthens traditional mutagenesis and high-throughput screening. In order to take care of the ever-growing population, it is seeking to limit the availability of food with limited land and water resources under a changing climate system. Traditional breeding has played an important role in increasing crop yields but takes long time and hard work. Later, transgenic technology came into being and played an important role in increasing crop yields. Therefore, more emphasis is now needed on crop improvement over non-GM technology. Nowadays, genome editing (GE) technique is also used in crop improvement projects because of its simplicity, robustness and high performance. Therefore, TILLING is a fast, simple, low cost, effective, widely available technique, independent of genotype and genome size. This review focuses on the prospect and potential use of the TILLING technique in crop improvement programs during valid genome editing.