Estimation of Vitamin A Concentration in Cattle Blood Based on Fluorescence With/Without Blood Cell Separation by Plasma Filter
摘要
“Japanese Black” is a breed of beef cattle endemic to Japan and has a propensity to produce marbled meat, which requires the regulation of the blood vitamin A, retinol. To control retinol levels in a healthy way, the fluorescence of whole blood, plasma and plasma with a blood cell separation filter of cattle was measured by Excitation Emission Matrix (EEM) fluorescence spectrophotometry to develop a blood retinol estimation technique that is simpler and faster than conventional chemical analysis. Actual blood retinol concentrations were determined by HPLC methods. The EEM of each sample was measured in the excitation 300–400 nm and fluorescence 320–550 nm range and the retinol concentration was estimated by PLS regression. R2Pred, and RMSEP for the whole blood were 0.91, 9.12, for plasma 0.94, 7.58, and for plasma with glass filter 0.92, 11.1, respectively.