A Novel Glutamate Three-Electrode System Based on Microelectrode Sensing Technology

Glutamate(Glu) plays an important role in brain function. The analysis of variations in Glu in physiological or pathological situations can be done using neuroelectrochemical sensing technology. Since the electrode is susceptible to interference in vivo and H2O2 is difficult to oxidize on carbon materials, it is necessary to improve the stability and sensitivity of the electrode when modifying the electrode. In this work, we first modified Pt nanoparticles and 3-thiopheneacetic acid(3-TAA) on the surface of carbon fiber microelectrode(CFME) in sequence, and finally fixed glutamate oxidase (GLOD) covalently on the surface of 3-TAA. So we successfully built the CFME/Pt-PTAA-GLOD sensor which is utilized to detect glutamate, providing a foundation for future neuroscience research.