Point of Care CRISPR/Cas Biosensing Technology: A Well Promising Management Strategies Towards the Possible COVID-19 Resurgence Caused by Hospital Contaminants

The Covid-19 pandemic caused by SARS-CoV-2 is highly transmissible and has significant public health concerns worldwide. It mainly spreads through respiratory droplets but there is a possibility of transmission through contaminated surfaces, especially in hospital settings, which has raised widespread concerns. CRISPR/Cas biosensing technology has potential in hospital settings to develop a portable device that can detect viral RNA on surfaces, equipment, and in the air. This can help identify areas that require more thorough cleaning, disinfection, and enable rapid isolation of infected patients. The chapter discusses the possibility of SARS-CoV-2 infection through hospital-contaminated surfaces, equipment, and air, and the management of it through an on-site testing system using CRISPR-based biosensors. The chapter discusses the application of CRISPR-based biosensors, including specialized identification components like Cas9, Cas12, and Cas13, which can be used with various readout techniques such as fluorescence, colorimetry, and lateral flow assay. The technology can be used in medical facilities to create a portable tool that can detect viral RNA on surfaces, tools, and the air. With further development and implementation, it can be a valuable tool for improving hospital safety and controlling the spread of the virus.