Application of Reference Materials in the Analysis of Medicinal Plant Raw Materials and Herbal Medicinal Products

In order to improve the standardization of medicinal plant raw materials and herbal medicinal products, new approaches to the analysis of plant objects are required. We substantiate the use of reference materials (RMs) in the development of assay procedures using a number of plant raw materials as an example. Biologically active compounds contained in these materials are studied in terms of their chemical composition, stability, and physicochemical properties. Active substances were determined by HPLC-UV. The results were processed using WinASPECT and Microsoft Excel 2016. Assay procedures were developed and validated for determining syringin in Syringa vulgaris bark and Eleutherococcus senticosus rhizomes and roots; rosavin and salidroside in Rhodiola rosea rhizomes and roots; arbutin in Arctostaphylos uva-ursi and Vaccinium vitis-idaea leaves; isosalipurposide in Helichrysum arenarium flowers; anthracene derivatives in Aloe arborescens leaves. Spectrophotometric methods for determining the amount of biologically active phenylpropanoids in terms of eleutheroside B (syringin) in medicinal plant raw materials and herbal medicinal products of Eleutherococcus senticosus and the amount of aralosides in Aralia mandschurica roots were proposed. The feasibility of using the RMs of syringin, rosavin and salidroside, araloside ammonium salts, arbutin, aloins A and B mixtures in assay procedures was substantiated.