Effect of Cardiolite on Enzyme Activity

Modern electrochemical research provides a good model for investigating reaction mechanisms and for characterizing enzymatic reactions in biological systems. The objective of this study is to determine the impact of a specific cardiolite on the activity of the catalase enzyme. Cardiolite (Tc-99m sestamibi) is a radiopharmaceutical diagnostic tool, which is used for diagnostic imaging in nuclear medicine, i.e. for the detection of coronary artery disease and assessment of myocardial function. Cardiolite accumulates in the heart muscle in proportion to the blood flow delivered by the coronary arteries. Additionally, it can be used to evaluate pathologies in breast and parathyroid gland tissues. The immobilization of Cardiolite was performed on a glassy carbon (GC) electrode. The immobilization process involved attaching the enzyme to the carrier to reduce its mobility and create a biosensor. All experiments were conducted using a three-electrode system employing cyclic voltammetry and chronoamperometry techniques. The results obtained were presented in terms of kinetic parameters. Based on these results, the Michaelis-Menten constant and the maximum reaction rate were calculated. The analysis revealed that the cardiolite exhibits a competitive type of inhibition on catalase activity.