Evaluation of Platelet-Enriched Plasma Antimicrobial Effect: In Vitro Study

Platelet-enriched plasma is autologous blood product, that contains platelets concentration two to three times above normal blood level. As such, PRP is a rich source of different bioactive molecules including grow factors, enzymes, cytokines, and chemokines. Beside its regenerative properties, a limited number of studies has proven that PRP can induce antimicrobial effect against single growing pathogens and biofilms. Aim of this study was to analyze PRP antimicrobial effect against three most common biofilm forming bacteria, including S. aureus, E. coli and P. aeruginosa. The antimicrobial property of PRP was evaluated after 24 h of incubation with selected bacteria in BHI media using spectrophotometer with a light source of 600 nm. To check whether PRP can inhibit bacterial biofilm formation, after 24 h incubation, tube screening test (TM) was applied. Bacteria treated with PRP and platelet poor plasma (PPP) were compared with untreated control, composed of bacteria growing spontaneously in BHI media. PRP produced strong growth inhibition in all tested bacteria when compared to bacteria treated with PPP and control group. Based on the obtained results it can be concluded that PRP can induce antimicrobial effect on S. aureus, P. aeruginosa and E. coli. PRP also reduced biofilm formation for P. aeruginosa and E. coli. However, there was no effect on S. aureus biofilm formation.