Characterization of the Chemical Substance Niacinamide

Niacinamide is a form of vitamin B3 and belongs to the group of B complex vitamins. It is used as a supplement and medicine, but also for dermocosmetic purposes. The aim of this work was to characterize the chemical substance niacinamide with a declared content of 99.0–101.0%, for the purpose of confirming the identification and the declared degree of purity. The substance was identified according to Ph. Yug. IV and according to Ph. Eur. 9, and the content according to Ph. Eur. 9, whereby the substance satisfied the necessary requirements. The identification was also performed by UV-VIS spectroscopy, where the absorption spectrum of the chemical substance was confirmed compared to the standard one, at 262 nm, and further tests were performed at wavelengths close to the absorption maximum. For the purpose of characterization, a series of 5 solutions of standard substance and 10 solutions of a chemical substance, concentration 0.04 mg/ml, was prepared and their absorption was recorded. For each wavelength at which the recording of the chemical substance was performed, a good recovery with RSD ≤ 2% was confirmed, and the average content of the chemical substance was 101.0%, which corresponds to the declared content. The test also confirmed the linearity of the UV-VIS method in the concentration range 0.005–0.05 mg/ml, with an acceptable correlation coefficient R2. The test confirmed the identity and content of the chemical in relation to the certified standard substance, and a satisfactory linearity method was developed that can be used in further tests of monocomponent preparations with niacinamide.