Multifrequency Bioimpedance Characterization of DNA Concentration: Sensitivity Analysis
摘要
In the past few decades, advancements have been made in the development of biosensors for the detection and analysis of Deoxyribonucleic Acid (DNA) and genes. The objective of this study was to explore Electrical Bioimpedance Spectroscopy (EBiS) for DNA concentration. EBiS patterns in the complex plane for different concentrations of salmon sperm DNA were obtained, and analyzed to identify potential sensitivity parameters for characterization and calibration curves design. According to the results, characteristic EBiS patterns are associated to the analyte vehicle and its parameters change as a function of the present DNA concentration. It seems possible to determine DNA concentration from measuring reactance (X) and resistance (R) at frequencies centered around 200 and 5 kHz, respectively. Exploration of X and R parameters at such frequencies seems pertinent to design calibration curves to estimate DNA concentration of 73 to 1200 ng/µL in a sample. Further studies are warranted to confirm the observations.