Preparation of Temperature-Sensitive Hollow Fiber Membrane and Its Detection as a Cell Carrier

NIPAAm has been widely used in cell culture and non-destructive desorption in recent years because it can change its surface configuration by changing temperature. In this study, NIPAAm was grafted onto the surface of the HFMs with cerium ammonium nitrate (CAN) as the initiator to prepare the thermosensitive HFMs. This material was used as a cell carrier for temperature sensitivity and biocompatibility assays to achieve non-destructive harvesting of cells from the surface of the HFMs. The morphology showed that poly (n-isopropylacrylamide) (PNIPAAm) was successfully grafted onto the HFMs. The results of water contact angle at different temperatures showed that the prepared samples have good temperature sensitivity. At 37 °C, PC12 cells adhered to and diffused well on the surface of PNIPAAm-g-HFMs. After cooling to 20 °C, the cells fell off the surface of the membrane in sheet form and retain more extracellular matrix proteins. PNIPAAm-g-HFMs in this study have good biocompatibility and can be better used as cell carriers in cell culture in vitro.