Antibacterial Effect of Injectable Platelet-Rich Fibrin Against Periodontal Pathogens

This study aimed to compare the antimicrobial activity of injectable platelet-rich fibrin (i-PRF) obtained from subjects with or without periodontal diseases against two pathogenic bacteria, Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg). I-PRF was prepared from blood samples of 60 individuals, including healthy subjects (n = 20), patients with gingivitis (n = 20), and patients with periodontitis (n = 20). The in vitro antibacterial effect of this platelet concentrate was evaluated using the agar diffusion test, a minimum inhibitory concentration (MIC) assay, and an antibacterial adhesion experiment. I-PRF exhibited a significantly better antibacterial effect against Pg than Aa within the periodontitis group, with a more expansive zone of inhibition and a lower MIC. Among the studied groups, i-PRF collected from the periodontitis group inhibited Aa and Pg significantly more in the agar diffusion test and had a lower MIC than i-PRF collected from the gingivitis and healthy groups. Although i-PRF reduced the adhesion of Aa and Pg and thus their ability to form a biofilm, the difference between groups of patients and the two pathogens was not significant. In conclusion, i-PRF exhibited an antibacterial effect against Aa and Pg in the zone of inhibition, MIC, and biofilm formation inhibition tests. However, in the periodontitis groups, i-PRF exhibited better antibacterial activity against Pg than Aa.