Investigate the Effect of Vitamin D3 on Osteogenic Differentiation of Mesenchymal Stem Cells Derived from Adipose Tissue (AT-Mscs) Under High D-Glucose Levels

Vitamin D3 plays a vital role in bone health, with low levels of vitamin D3 being related to skeletal fragility, fractures, and metabolic disorders such as diabetes. A correlation between diabetes mellitus and osteoporosis is attracting considerable interest, and research to find the prevention and treatment is gradually being studied. In this study, we investigated the effect of vitamin D3 on the osteogenic differentiation of mesenchymal stem cell-derived adipose tissue (AT-MSCs) under high D-glucose concentrations. Differentiated AT-MSCs were analyzed by Alizarin Red S staining and optical density measurement. The results have shown that Vitamin D3 has slightly increased the osteogenic differentiation of AT-MSCs under high D-glucose conditions. Therefore, the data partially explain an aspect of osteoporosis-related diabetes pathological mechanism, which can be applied for further research on the treatment of osteoporosis in diabetic patients, as well as an in vitro model for pre-clinical drug screening that can be used instead of an in vivo model.