The Effect of 10% Platelet-Rich Plasma on In-Vitro Wound Healing Ability of Adipose Tissue-Derived Mesenchymal Stem Cells Under High D-Glucose Conditions

Diabetic patients display increased risk of diabetic foot ulcer and chronic wound, mainly caused by the persistence of hyperglycemia. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) and platelet rich plasma (PRP) have shown relevant advantages for wound healing by their ability to stimulate cell proliferation, migration, secrete growth factors and cytokines to accelerate the resolution of wound. In this study, we investigated the effect of PRP on the proliferation and wound healing in-vitro of AT-MSCs under high D-glucose conditions. PRP was activated by Thrombin and Calcium chloride. The cell proliferation was determined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay for 10 days. In-vitro scratch assay was used to examine the healing ability of AT-MSCs under the influence of high D-glucose concentrations. The results show that the doubling time of AT-MSCs cultured with PRP was significantly higher than those with FBS. Moreover, the wound healing ability in-vitro was induced significantly in PRP-treated AT-MSCs under high D-glucose conditions. Our research demonstrates the potential of PRP on the inducing wound-healing capacity of AT-MSCs under the impact of high D-glucose levels. This work gives a better knowledge of the influence of PRP on the activities of AT-MSCs under high D-glucose conditions for future applications of AT-MSCs in the treatment of diabetes complications.