Investigation of Codonopsis Javanica Root Extract on Open Wound Model: In Vitro and In Vivo Studies

From ancient times, Codonopsis javanica root (CJR) has been used in Eastern traditional medicine to treat body weaknesses. CJR was dried and crushed into powder before being extracted using the reflux method with modified parameters such as solvent concentration, material-solvent ratio, and reaction time to obtain the total phytochemical constituents inside. The extraction was then loaded into Carbopol 934 to form a topical hydrogel. The DPPH assay indicated that the extraction has outstanding antioxidant properties. In vitro cell proliferation demonstrated that the presence of CJR extract helps accelerates cell growth and migration. However, an excessive quantity might reduce cell viability. Besides, in vivo evaluation in mice models also indicated the enhancement in epithelialization and regeneration process. Hence, this study introduced a topical drug used to promote healing rates with the extract concentration of 2.5 mg/ml in the sample was deemed optimal for further potential applications in open wound treatment.