Distribution In Vivo

Distribution studies with radiolabeled test substances in animals are an important part of the drug development process. Traditional routine methods used for these studies are quantitative tissue distribution studies (QTD) and alternatively whole-body autoradiography (WBA) with detection of the radioactivity in whole-body sections on X-ray film (Baker, Autoradiography: a comprehensive overview, microscopy handbooks 18. Oxford University Press/Royal Microscopical Society, Oxford/New York, 1989). WBA is a qualitative detection method with a very high local resolution which includes all organs and many small substructures.