Antimicrobial Activities of Organic Solvent Extracts of Citrus aurentifolia, Eucalyptus globulus and Jasmine grandiflorium Against Certain Microorganisms

Bioassays for antimicrobial activities were carried out using peels, leaves & flowers of Citrus aurentifolia, Eucalyptus globulus and Jasmine grandiflorium respectively. Various organic solvents extract of these plants were evaluated for antimicrobial activity against clinically important organism viz. Escherichia coli ATCC25922, Klebsiella pneumoniae NCIM2719, Pseudomonas aeruginosa ATCC27853 and Saccharomyces cerevisiae ATCC9763. The plants Extracts were isolated by means of a Soxhlet device and as per accordance to polarity of solvents. The in vitro antimicrobial activity was performed by agar well diffusion method The Minimum Inhibitory Concentration MIC of the plant extracts were 20 µl. The organic solvents extract of all the plants were active against all the investigated bacterial strains while ethanolic extracts were showing considerable activity. Accelerated gradient chromatography (AGC) gave fractions of the extract that were also active as antimicrobial crude extract fractions.