The Comprehensive Inventory of Mindfulness Experiences (CHIME) represents a 37-item self-assessment instrument that encompasses eight dimensions of dispositional mindfulness previously identified through research: (1) awareness of internal experiences, (2) awareness of external experiences, (3) acting with awareness, (4) accepting, nonjudgmental attitude, (5) nonreactive decentering, (6) openness to experience, (7) awareness of thoughts’ relativity, and (8) insightful understanding. The CHIME emerged from efforts to evaluate these mindfulness dimensions within one comprehensive instrument. Additionally, guidelines for item construction were established based on limitations observed in earlier mindfulness assessment tools, seeking to enhance the instrument’s reliability and validity. In contrast to items from previously constructed questionnaires, the phrasing was refined to be more tangible and precise, connected to daily-life situations, while minimizing vagueness. Each of the eight subscales contained affirmatively phrased items. Moreover, item construction deliberately avoided specialized terminology and language characteristic of mindfulness-based programs or contemplative practices, enabling application across general populations. The CHIME originated in German and underwent English validation shortly after. The factorial organization, reliability, and validity were confirmed through a community sample (n = 298) and participants in the Mindfulness-Based Stress Reduction course (n = 161). Factor-analytic methods confirmed an 8-factor organization. This organization received further testing in an additional confirmatory sample (n = 202). The instrument and subscales demonstrated strong internal consistency and test–retest reliability. Examination of measurement invariance across individual items among groups varying in age, gender, contemplative practice experience, and symptom severity indicated no systematic variations in item interpretation. Indices reflecting construct validity, criterion validity, and incremental validity, alongside sensitivity to change, were consistently satisfactory or better. This research, along with follow-up investigations, has shown sound psychometric characteristics in the original German version as well as Chinese, Dutch, English, and additional language adaptations. Subsequent developments have involved enhancing the original scales’ measurement characteristics through Rasch analysis, creating abbreviated versions, and developing an experience sampling variant. The CHIME serves as a self-report instrument with strong psychometric characteristics addressing multiple mindfulness aspects pertinent to mindfulness theory, research, behavioral health applications, and therapeutic interventions.