Nowadays, the efforts of biotechnologists are rewarded in increasing microbial metabolite routes by producing de novo structured lipids, namely conjugated linoleic acids (CLA). These types of dietary lipids have been found to exhibit multifunctional biological activities owing to some particular isomer types of CLAs. Although trans-10 and cis-12 are noted to be the most active isomers out of 50 types of isomers, the de novo isomers cis-7, cis-9 CLA and trans-7, trans-9 CLA are expected to be among the most effective ones because of their least isomerizing effects. These types of isomers are the throughput of fermentation byproducts initiating via partial hydrogenation and they can be used as a step-limiting rate for complete biohydrogenation or reduced hydrogenation. The surprising key finding is that the critical concentration of bioconverted linoleic acid (LA), which is found to be highly dependent on the formed CLA, where CLA% should be less than LA% (CLA < LA), this chemistry notification was attributed to multiple reasons such as (1) the possible occurrence of gene tolerance and immediate response to inflammatory signs and (2) the co-attachments of nitric oxide species to CLA isomers, which may render the resulting CLA on forming hetero-N-compounds having a direct response to inflammatory production and further cytokine release. Therefore, in order to tune the fermentation process in particular biohydrogenation, it is important to take into consideration the levels of LA, CLA, and stearic acid, which is considered a step-limiting rate for carbonyl-chain elongation. Overall, several mechanisms are implicated in triggering CLA positional isomers; in particular, the formation of conjugated dienes and trienes such as transesterification, lipolysis, biohydrogenation, reduced hydrogenation, lipid biosynthesis, gene hybridization and amplifications, gene delivery systems, and formation of a mitogen region or eradication of a region via fermentation. All these mechanisms are the reason behind the construction of particular isomers capable of combating the burden of diseases via significant enhancement in the repair elements, mitochondrial signaling, and potentiating both protein and gene machineries via gene-guiding therapy and gene-labeling strategies for empowering disease traceability and their in vivo radio-imaging.