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The hypersensitive response (HR) represents a critical antiviral pathway in plants that can be leveraged for therapeutic screening via half-leaf spot assay. Attenuation of HR-induced necrotic lesions serves as a quantitative readout of antiviral efficacy. Here we present an integrated protocol combining: (1) rhizosphere bacteria isolation from asymptomatic plants in virus-infecting fields via serial dilution plating; and (2) quantitative antiviral assessment through necrotic lesion analysis. This comprehensive approach identifies microbial immunomodulators with potential for sustainable plant virus control.

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Enhancing Plant Antiviral Resistance with Microbial Immunomodulators: A Half-Leaf Spot Screening Method

  • 实验方案

  • 发表日期：

    2026年

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  • Chao Ma,
  • Kelei Han,
  • Dankan Yan

摘要

The hypersensitive response (HR) represents a critical antiviral pathway in plants that can be leveraged for therapeutic screening via half-leaf spot assay. Attenuation of HR-induced necrotic lesions serves as a quantitative readout of antiviral efficacy. Here we present an integrated protocol combining: (1) rhizosphere bacteria isolation from asymptomatic plants in virus-infecting fields via serial dilution plating; and (2) quantitative antiviral assessment through necrotic lesion analysis. This comprehensive approach identifies microbial immunomodulators with potential for sustainable plant virus control.