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High-temperature treatment (thermotherapy) inhibits viral RNA synthesis, restricting viral particle movement into the apical meristem and enabling virus elimination when applied for optimized durations. Combined thermotherapy and meristem culture enhances the production of virus-free germplasm. Here, we describe optimized protocols for thermotherapy, meristem culture, and transplantation of virus-free pear plants.

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Virus Elimination by Thermotherapy Combined with Meristem Culture

  • 实验方案

  • 发表日期：

    2026年

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  • Ni Hong,
  • Guoping Wang

摘要

High-temperature treatment (thermotherapy) inhibits viral RNA synthesis, restricting viral particle movement into the apical meristem and enabling virus elimination when applied for optimized durations. Combined thermotherapy and meristem culture enhances the production of virus-free germplasm. Here, we describe optimized protocols for thermotherapy, meristem culture, and transplantation of virus-free pear plants.