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Phosphatidic acid (PA), a key signaling molecule in plant immunity, activates MAPK-mediated and reactive oxygen species (ROS)-mediated defense responses. Exogenous application of PA significantly reduces Potato virus Y (PVY) genomic RNA accumulation and alleviates disease symptoms in Nicotiana benthamiana, highlighting its potential as an immune elicitor for crop disease management. This chapter provides detailed protocols for PA solution preparation, MAPK pathway activation analysis (by Western blot and qPCR), and virus quantification to guide PA application for broader plant virus control in crops.

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Phosphatidic Acid Application to Enhance Plant Virus Resistance

  • 实验方案

  • 发表日期：

    2026年

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  • Jiayu Lin,
  • Wei Zhang,
  • Hongtao Zhang,
  • Bingjian Sun,
  • Hangjun Sun

摘要

Phosphatidic acid (PA), a key signaling molecule in plant immunity, activates MAPK-mediated and reactive oxygen species (ROS)-mediated defense responses. Exogenous application of PA significantly reduces Potato virus Y (PVY) genomic RNA accumulation and alleviates disease symptoms in Nicotiana benthamiana, highlighting its potential as an immune elicitor for crop disease management. This chapter provides detailed protocols for PA solution preparation, MAPK pathway activation analysis (by Western blot and qPCR), and virus quantification to guide PA application for broader plant virus control in crops.