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The establishment of primary cells from fresh tissue is a widely used method for investigating human tissue in vitro. The skin harbors different cell populations in the dermis and the hair follicle, which can be isolated for downstream analysis. Here, we describe the isolation of four dermal fibroblast populations from human haired skin and their maintenance in culture. The four cell population isolations described are the papillary dermal fibroblast cells, the reticular dermal fibroblast cells, the hair follicle dermal sheath cells, and the hair follicle dermal papilla cells.

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Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle

  • 实验方案

  • 发表日期：

    2026年

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  • Rachael Williams,
  • M. Julie Thornton

摘要

The establishment of primary cells from fresh tissue is a widely used method for investigating human tissue in vitro. The skin harbors different cell populations in the dermis and the hair follicle, which can be isolated for downstream analysis. Here, we describe the isolation of four dermal fibroblast populations from human haired skin and their maintenance in culture. The four cell population isolations described are the papillary dermal fibroblast cells, the reticular dermal fibroblast cells, the hair follicle dermal sheath cells, and the hair follicle dermal papilla cells.