This protocol focuses on the extraction and determination of neuroprotective capacity (neuromodulators) of plant parts from Bacopa monnieri and Sterculia setigera. Both plants are traditionally recognized for their neuroprotective properties. The protocol includes extraction, in vitro and in vivo assessment of neuroprotective effects, behavioral and biochemical analyses, and molecular assays to evaluate neuroprotection against induced neurotoxicity. The methodology outlines systematic identification and characterization of neuroprotective compounds from these plant part extracts.