Saponins, natural glycosides found in a variety of plants, are known for their unique ability to form stable foams due to their surfactant-like structure. These bioactive compounds hold immense potential in the food, pharmaceutical, and cosmetic industries. However, the extraction of saponins can be challenging due to their sensitivity to heat and the lengthy processes associated with conventional methods. In this chapter, we present a detailed protocol for extracting saponins from plant leaves using advanced techniques—ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE). These non-conventional methods offer significant advantages, including reduced solvent use, shorter extraction times, and improved yields while preserving the integrity of heat-sensitive compounds. This protocol can be applied across various plant species, making it a versatile tool for researchers looking to efficiently extract saponins for diverse applications. By leveraging these innovative techniques, this method enhances both the practicality and sustainability of saponin extraction.