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Microbiome-integrated Caenorhabditis elegans cultivation methods enable investigation of host–microbiome interactions in the context of space-relevant stresses using three key innovations: introduction of live bacterial communities replacing chemically defined media, implementation of auxin-inducible degradation systems to prevent progeny production, and development of complementary hardware platforms. Polyethylene bags provide gas-permeable cultivation environments for large populations with complex microbiomes supporting downstream molecular analyses, while NemaCapsules with micropillar arrays and passive culturing chambers allow real-time phenotypic assessment through on-orbit imaging, transforming our ability to correlate molecular signatures with physiological outcomes in microgravity.

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Defining Microbiome Impact on Host Physiology During Spaceflight Using Caenorhabditis elegans

  • 实验方案

  • 发表日期：

    2026年

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  • Dana Blackburn,
  • Bushra Rahman,
  • Atiyya P. Saroyia,
  • Audrey J. Parish,
  • Monica Driscoll,
  • Nathaniel J. Szewczyk,
  • Siva A. Vanapalli,
  • Buck S. Samuel

摘要

Microbiome-integrated Caenorhabditis elegans cultivation methods enable investigation of host–microbiome interactions in the context of space-relevant stresses using three key innovations: introduction of live bacterial communities replacing chemically defined media, implementation of auxin-inducible degradation systems to prevent progeny production, and development of complementary hardware platforms. Polyethylene bags provide gas-permeable cultivation environments for large populations with complex microbiomes supporting downstream molecular analyses, while NemaCapsules with micropillar arrays and passive culturing chambers allow real-time phenotypic assessment through on-orbit imaging, transforming our ability to correlate molecular signatures with physiological outcomes in microgravity.