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Geographical position sequencing (Geo-seq), combining transcriptome sequencing and laser capture microdissection, enables comprehensive learning of gene expression within the spatial context. Geo-seq is robust for in-depth gene expression analyses from tissue section at specific position coordinate across various tissue types. The abundance of specific transcripts and the whole transcriptomic architecture can be determined following region-specific laser capture microdissection, RNA extraction, and cDNA preparation. Generally, Geo-seq enables high-throughput profiling of the transcriptomic architecture encompassing over 8000 genes from a single sampled spot.

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Transcriptomic Analysis from Cryosectioned Tissue Samples Using Laser Capture Microdissection-Based Geo-Seq

  • 实验方案

  • 发表日期：

    2026年

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  • Xianfa Yang,
  • Yingying Chen,
  • Yun Yang,
  • Naihe Jing

摘要

Geographical position sequencing (Geo-seq), combining transcriptome sequencing and laser capture microdissection, enables comprehensive learning of gene expression within the spatial context. Geo-seq is robust for in-depth gene expression analyses from tissue section at specific position coordinate across various tissue types. The abundance of specific transcripts and the whole transcriptomic architecture can be determined following region-specific laser capture microdissection, RNA extraction, and cDNA preparation. Generally, Geo-seq enables high-throughput profiling of the transcriptomic architecture encompassing over 8000 genes from a single sampled spot.