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Chromosomal instability can be measured directly via metaphase chromosome analysis or indirectly via an alternative, simpler method known as the micronucleus (MN) test. Micronuclei (MNi) form when chromosomal fragments or entire chromosomes fail to segregate correctly during cell division. They are then enclosed by a membrane and appear in the cytoplasm of the daughter cells during interphase. As this test is quicker and easier to perform than metaphase chromosome analysis, it is often used as an endpoint for genotoxicity. By combining the MN test with fluorescence in situ hybridisation (FISH), the chromosomal content of MNi can be characterized. Understanding the origin and content of MNi using FISH is essential for the proper interpretation of this cytogenetic endpoint.

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Micronucleus FISH

  • 实验方案

  • 发表日期：

    2026年

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  • Galina Hovhannisyan,
  • Tigran Harutyunyan,
  • Rouben Aroutiounian,
  • Thomas Liehr

摘要

Chromosomal instability can be measured directly via metaphase chromosome analysis or indirectly via an alternative, simpler method known as the micronucleus (MN) test. Micronuclei (MNi) form when chromosomal fragments or entire chromosomes fail to segregate correctly during cell division. They are then enclosed by a membrane and appear in the cytoplasm of the daughter cells during interphase. As this test is quicker and easier to perform than metaphase chromosome analysis, it is often used as an endpoint for genotoxicity. By combining the MN test with fluorescence in situ hybridisation (FISH), the chromosomal content of MNi can be characterized. Understanding the origin and content of MNi using FISH is essential for the proper interpretation of this cytogenetic endpoint.