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Multicolor FISH using whole chromosome painting and FISH-banding with partial chromosome painting probes are not suitable for characterizing small centromeric and pericentromeric rearrangements for technical reasons. Therefore, methods have been developed to characterize the different human centromeres and further probe sets to investigate the centromere-proximal regions of human chromosomes. In this chapter, such centromere-specific multicolor (cenM) and subcentromere-specific (subcenM) FISH techniques are presented. The setup and use of these probe sets are described.

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Centromere-Directed M-FISH Approaches (cenM-FISH)

  • 实验方案

  • 发表日期：

    2026年

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  • Thomas Liehr

摘要

Multicolor FISH using whole chromosome painting and FISH-banding with partial chromosome painting probes are not suitable for characterizing small centromeric and pericentromeric rearrangements for technical reasons. Therefore, methods have been developed to characterize the different human centromeres and further probe sets to investigate the centromere-proximal regions of human chromosomes. In this chapter, such centromere-specific multicolor (cenM) and subcentromere-specific (subcenM) FISH techniques are presented. The setup and use of these probe sets are described.