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Peripheral blood, amniotic fluid, chorion, and fibroblasts are the most commonly used tissues for chromosome analysis. All four are relatively easy to obtain, can be easily brought into short-term culture, and metaphase preparations can be made within a relatively short time. Such metaphase preparations, as well as the large quantities of previously superfluous interphase nuclei in cytogenetic preparations, are very well suited for FISH analysis. All types of FISH probes can be used to analyze metaphase preparations, while interphase nuclei can only be analyzed with satellite and locus-specific probes for routine purposes. Since the quality and quantity of metaphases are important in research and routine diagnostics, the chromosome preparation procedure is described in more detail here. In addition, a standard two-color FISH procedure is described, which offers the possibility of enhancing the DAPI (4′,6-diamidino-2-phenylindole) banding pattern during cultivation with BrdU (bromdesoxyuridine).

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Pre- and Postnatal Diagnostics and Research on Peripheral Blood, Bone Marrow Chorion, Amniocytes, and Fibroblasts

  • 实验方案

  • 发表日期：

    2026年

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  • Anja Weise,
  • Thomas Liehr

摘要

Peripheral blood, amniotic fluid, chorion, and fibroblasts are the most commonly used tissues for chromosome analysis. All four are relatively easy to obtain, can be easily brought into short-term culture, and metaphase preparations can be made within a relatively short time. Such metaphase preparations, as well as the large quantities of previously superfluous interphase nuclei in cytogenetic preparations, are very well suited for FISH analysis. All types of FISH probes can be used to analyze metaphase preparations, while interphase nuclei can only be analyzed with satellite and locus-specific probes for routine purposes. Since the quality and quantity of metaphases are important in research and routine diagnostics, the chromosome preparation procedure is described in more detail here. In addition, a standard two-color FISH procedure is described, which offers the possibility of enhancing the DAPI (4′,6-diamidino-2-phenylindole) banding pattern during cultivation with BrdU (bromdesoxyuridine).