Immunoprecipitation of Protein Complexes from Trypanosoma brucei

实验方案
发表日期：

2026年

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Aleksandra Ciszek,
Midori Ishii,
Bungo Akiyoshi

摘要

Immunoprecipitation enables the isolation of protein complexes. Here we describe a method to purify YFP-tagged proteins from Trypanosoma brucei. This method has been optimized for kinetochore proteins but can readily be adapted to other proteins.

Immunoprecipitation enables the isolation of protein complexes. Here we describe a method to purify YFP-tagged proteins from Trypanosoma brucei. This method has been optimized for kinetochore proteins but can readily&#xa0;be adapted to other proteins.

Immunoprecipitation of Protein Complexes from Trypanosoma brucei

摘要

Immunoprecipitation enables the isolation of protein complexes. Here we describe a method to purify YFP-tagged proteins from Trypanosoma brucei. This method has been optimized for kinetochore proteins but can readily be adapted to other proteins.