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Genetically encoded calcium indicators (GECIs) are proteins used to monitor calcium ion (Ca2+) changes in living cells. They can be targeted to the cytosol or to organelles for continuous measurements in vivo. In this chapter, we present an overview of the methods that our lab used to detect Ca2+ changes in Trypanosoma cruzi elicited by stimulation of Ca2+ channels in the plasma membrane or in intracellular organelles, including selection of adequate GECIs, their transfection and selection, and their use for microscopic and fluorometric analysis.

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Imaging Ca2+ Signaling in Trypanosoma cruzi with Genetically Encoded Ca2+ Indicators

  • 实验方案

  • 发表日期：

    2026年

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  • Guozhong Huang,
  • Mayara Bertolini,
  • Roberto Docampo

摘要

Genetically encoded calcium indicators (GECIs) are proteins used to monitor calcium ion (Ca2+) changes in living cells. They can be targeted to the cytosol or to organelles for continuous measurements in vivo. In this chapter, we present an overview of the methods that our lab used to detect Ca2+ changes in Trypanosoma cruzi elicited by stimulation of Ca2+ channels in the plasma membrane or in intracellular organelles, including selection of adequate GECIs, their transfection and selection, and their use for microscopic and fluorometric analysis.