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This chapter outlines the assembly and annotation of mitochondrial genomes and transcriptomes of kinetoplastids and diplonemids using high-throughput sequencing data. The mitochondrial genomes of these protists are highly atypical, characterized by multipartition and extensive RNA editing. These complexities pose significant challenges for genome assembly, gene prediction, and transcript reconstruction. Here, we describe analytical strategies developed in our laboratories that address these issues.

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Assembly and Annotation of Kinetoplastid and Diplonemid Mitochondrial Genomes

  • 实验方案

  • 发表日期：

    2026年

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  • Evgeny S. Gerasimov,
  • Matus Valach,
  • Gertraud Burger,
  • Kristína Záhonová,
  • Vyacheslav Yurchenko

摘要

This chapter outlines the assembly and annotation of mitochondrial genomes and transcriptomes of kinetoplastids and diplonemids using high-throughput sequencing data. The mitochondrial genomes of these protists are highly atypical, characterized by multipartition and extensive RNA editing. These complexities pose significant challenges for genome assembly, gene prediction, and transcript reconstruction. Here, we describe analytical strategies developed in our laboratories that address these issues.