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Marine diplonemids (Diplonemidae and Hemistasiidae), once considered difficult to culture, can now be introduced into stable axenic cultures and grown to high densities. This chapter provides detailed protocols on establishing clonal cultures from environmental cells, their axenization and routine maintenance, as well as instructions on mid- and large-scale cultivation, estimation of cell density, and cryopreservation of selected species.

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Isolation and Cultivation of Diplonemids

  • 实验方案

  • 发表日期：

    2026年

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  • Daria Tashyreva,
  • Galina Prokopchuk,
  • Orsola Iorillo,
  • Matus Valach,
  • Ingrid Škodová-Sveráková,
  • Dong-Woo Shin,
  • James A. Letts,
  • Gertraud Burger,
  • Julius Lukeš,
  • Drahomíra Faktorová

摘要

Marine diplonemids (Diplonemidae and Hemistasiidae), once considered difficult to culture, can now be introduced into stable axenic cultures and grown to high densities. This chapter provides detailed protocols on establishing clonal cultures from environmental cells, their axenization and routine maintenance, as well as instructions on mid- and large-scale cultivation, estimation of cell density, and cryopreservation of selected species.