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Calcium sensors as tools to visualize calcium dynamics have significantly advanced our understanding of calcium signaling, but visualization of calcium decoding remains a major challenge. Towards this end, we developed the CPKaleon reporter for monitoring conformational changes of the calcium sensor-decoder family of calcium-dependent protein kinases (CPK). It enables the real-time visualization of CPK activation and inactivation in response to specific treatments. Here, detailed protocols for the in vitro characterization and in vivo imaging of CPKaleon are presented.

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Visualization of Calcium Decoding via a FRET-Based Reporter for Calcium-Decoder Conformation

  • 实验方案

  • 发表日期：

    2026年

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  • Anja Liese,
  • Sarah Lederer,
  • Bernadette Eichstädt,
  • Tina Romeis

摘要

Calcium sensors as tools to visualize calcium dynamics have significantly advanced our understanding of calcium signaling, but visualization of calcium decoding remains a major challenge. Towards this end, we developed the CPKaleon reporter for monitoring conformational changes of the calcium sensor-decoder family of calcium-dependent protein kinases (CPK). It enables the real-time visualization of CPK activation and inactivation in response to specific treatments. Here, detailed protocols for the in vitro characterization and in vivo imaging of CPKaleon are presented.