Eye development is a complex process that begins during the first stages of embryonic development; during its formation, a series of cell proliferation, migration, and differentiation events occur. A frequently used laboratory method is tissue staining with two dyes known as hematoxylin–eosin (H&E). H&E staining allows us to observe the arrangement of the cell layers and the formation of characteristic structures. It is fundamental for research in developmental biology since it facilitates a comprehensive analysis of the formation of organs and systems. In this chapter, we will describe the procedure for acquiring H&E-stained images of the embryonic eye structure, specifically focusing on the retina. This technique serves as a fundamental tool for understanding ocular morphogenesis, organogenesis, and structural modifications resulting from experimental interventions.