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Flow cytometry and fluorescence-activated cell sorting (FACS) techniques have significantly advanced the characterization of adipocyte precursor cell (APC) populations. They allow immuno-phenotyping, quantification, and isolation of distinct populations, which critical for understanding adipose tissue development and homeostasis. Here, we describe the identification and purification of APCs using flow cytometry, FACS, and cytometry by time-of-flight (CyTOF), defined by previously established surface marker profiles. In addition, we describe the mouse models and whole adipose tissue visualization techniques that will enable us to characterize the cellular origin and plasticity of APCs.

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Characterization of Adipocyte Precursor Cells (APCs) by Lineage Tracing, Flow Cytometry, and Cytometry Time of Flight (CyTOF)

  • 实验方案

  • 发表日期：

    2026年

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  • Qiwei Zhang,
  • Nathaniel Vo,
  • Ju Hee Lee,
  • Azadeh Yeganeh,
  • Kafi Ealey,
  • Hoon-Ki Sung

摘要

Flow cytometry and fluorescence-activated cell sorting (FACS) techniques have significantly advanced the characterization of adipocyte precursor cell (APC) populations. They allow immuno-phenotyping, quantification, and isolation of distinct populations, which critical for understanding adipose tissue development and homeostasis. Here, we describe the identification and purification of APCs using flow cytometry, FACS, and cytometry by time-of-flight (CyTOF), defined by previously established surface marker profiles. In addition, we describe the mouse models and whole adipose tissue visualization techniques that will enable us to characterize the cellular origin and plasticity of APCs.